Pennsylvania Route 241 (PA 241) is a 23 mile long state highway in the U.S. state of Pennsylvania. The route runs from Pennsylvania Route 441 in Conoy Township, Lancaster County northeast to Pennsylvania Route 72 in the city of Lebanon in Lebanon County.

PA 241 heads northeast from Pennsylvania Route 441 on Bainbridge Road. It passes through West Donegal Township and enters Elizabethtown. It follows Bainbridge Street to a left turn onto Masonic Drive. It then turns right onto West High Street and follows it to Market Street (Pennsylvania Routes 230 and 743). PA 241 turns left on Market Street, forming a three-way concurrency with PA 230 and PA 743. PA 241 and PA 743 split from PA 230 by a right onto Linden Avenue for a short distance and then a left onto North Hanover Street. At the edge of Elizabethtown, the two routes split and PA 241 continues northeast on Mount Gretna Road.

PA 241 enters Mount Joy Township and crosses over the Pennsylvania Route 283 expressway with no interchange. It then enters South Londonderry Township in Lebanon County, where the name changes to Elizabethtown Road, and it crosses under the Pennsylvania Turnpike (Interstate 76). It then continues east and intersects the eastern terminus of Pennsylvania Route 341 and then intersects Pennsylvania Route 117 in Colebrook. PA 241 turns north for a brief concurrency with PA 117 on Mount Wilson Road, with PA 117 eventually heading northwest on South Forge Road. PA 241 continues north through the mountain community of Mt. Wilson before entering the Lebanon Valley in South Annville Township, where it intersects U.S. Route 322.

PA 241 turns east for a brief concurrency with U.S. Route 322, where it intersects the southern terminus of Pennsylvania Route 934 and enters West Cornwall Township. It then heads northeast on Colebrook Road and passes through North Cornwall Township. It then enters the city of Lebanon where it ends at Pennsylvania Route 72.