In the U.S. state of Ohio, Interstate 76 (I-76) runs for 82 miles (132 km) from Interstate 71 south of Cleveland to the Pennsylvania state line south of Youngstown. Once numbered Interstate 80S, Interstate 76 now comprises the easternmost leg of the Ohio Turnpike.

Interstate 76 begins at an interchange with Interstate 71 east of Lodi, Ohio cosigned with US 224. The interstate travels east across Medina County before entering Summit County. In Akron, Interstate 76 runs concurrently with Interstate 77 for approximately 3 miles before it becomes its own road again. Interstate 76 continues east through the Akron suburbs and runs a rural route to just west of Youngstown, where it intersects with Interstate 80 and the Ohio Turnpike. Interstate 76 eastbound traffic exits the freeway alignment to join the Ohio Turnpike. Travelers who remain on the freeway alignment transfer to Interstate 80 which leads through the northern Youngstown suburbs and into Pennsylvania via the Keystone Shortway. Interstate 76 travels southeast once joining the Ohio Turnpike as it directly connects to the Pennsylvania Turnpike.

As of 2010, the toll to travel the Interstate 76 portion of the Ohio Turnpike is $1.50. Motorists who use EZ-Pass pay a discounted rate of $1.