Pennsylvania Route 41 (PA 41) is a major 22-mile (35 km) long state highway located in southeastern Pennsylvania. The southern terminus of the route is at the Delaware state line in New Garden Township, where it continues as Delaware Route 41 (DE 41). The northern terminus is at U.S. Route 30 just north of Gap.

PA 41, also known as Gap-Newport Pike (or Newport-Lancaster Pike), serves as the primary thoroughfare between Lancaster's eastern suburbs and Wilmington, Delaware; as the original southern terminus of PA / DE 41 was in Newport, the highway's nickname persists. Frequent accidents have motivated PennDOT's many improvements along its length, as well as the addition of signage reminding motorists to drive safely.

PA 41 begins at the Delaware border in Kennett Township, Chester County, where the road continues into that state as DE 41. From the state line, the route heads northwest on two-lane undivided Gap Newport Pike, passing through farmland with some development. The road enters New Garden Township and comes to an interchange with Limestone Road, which heads south to the Delaware border and becomes DE 7. PA 41 continues northwest through more rural areas with some homes and businesses. Then route heads into Avondale, where it crosses an East Penn Railroad line and comes to an intersection with Baltimore Pike. Here, the road becomes Pennsylvania Avenue and passes through wooded residential areas. PA 41 leaves Avondale for London Grove Township and becomes Gap Newport Pike again, passing near homes and farmland before Baltimore Pike heads to the west. The route continues through commercial areas before coming to an interchange with the US 1 freeway.

Following this, the road runs through agricultural areas with some woods and homes, reaching an intersection with PA 841 in the community of Chatham. PA 41 continues through more rural areas and enters Londonderry Township, where it comes to a junction with PA 926. A short distance later, the road reaches a junction with the northern terminus of PA 796. The route runs through more farmland with some woodland and residences, crossing into West Fallowfield Township. Here, PA 41 comes to the community of Cochranville, where it crosses PA 10. After Cochranville, the road heads through more open farmland with some patches of woods and homes. The route briefly passes through a corner of West Sadsbury Township before it enters Atglen and comes to an intersection with PA 372. Following this, PA 41 heads into wooded areas and comes to a bridge over Amtrak's Philadelphia to Harrisburg Main Line. The road curves northwest and crosses back into West Sadsbury Township, passing through a mix of farm fields and development.

PA 41 crosses Pine Creek into Sadsbury Township in Lancaster County and heads west past homes and businesses as it bypasses Christiana to the north. The road curves to the north-northwest and passes through a mix of farms and woods with some commercial development a short distance to the east of the Amtrak line. The route heads north and crosses into Salisbury Township, where it enters the residential community of Gap and comes to an intersection with the eastern terminus of PA 741. PA 41 heads into business areas and comes to its northern terminus at a junction with US 30.