Minnesota State Highway 16 is a two-lane highway in southeast Minnesota, which runs from its interchange with Interstate Highway 90 in Dexter and continues east to its eastern terminus at its intersection with U.S. Highways 14 and 61 in La Crescent.

The route was designated as a National Scenic Byway in 2003 as the Historic Bluff Country Scenic Byway. The roadway follows the route of old U.S. Highway 16.

Highway 16 is 88 miles (142 km) in length.

State Highway 16 serves as an east–west route in southeast Minnesota between Dexter, Spring Valley, Preston, Rushford, Houston, and La Crescent. Highway 16 parallels Interstate 90 throughout its route in southeast Minnesota.

The route passes through the Richard J. Dorer Memorial Hardwood State Forest in Fillmore and Houston counties.

Forestville Mystery Cave State Park is located near Highway 16 in Fillmore County. The park is located between Spring Valley and Preston.

The eastern terminus for Highway 16 is its junction with U.S. Highways 14 and 61 in La Crescent.

The western terminus for the route is its interchange with Interstate 90 in Dexter.

State Highway 16 was designated in 1980. The route was originally part of old U.S. Highway 16 from 1926 to 1980.

U.S. 16 was mostly replaced by Interstate Highway 90 within Minnesota.

State Highway 16 from Dexter to La Crescent is the only remaining section of this former U.S. route that exists as a trunk highway separate from Interstate 90.

Until 2008, the highway was concurrent with U.S. 14 / 61 for one mile through La Crescent. After crossing the Mississippi River Bridge and the Wisconsin state line, old U.S. 16 continues on as Wisconsin Highway 16.

The only section of U.S. Highway 16 that remains in the present day is the east–west route between Rapid City, South Dakota and Yellowstone National Park in Wyoming.

Mileposts are retained from old U.S. 16.