State Route 289 (SR 289) is a state highway in Humboldt County, Nevada. The route serves the town of Winnemucca, and is cosigned with Interstate 80 Business for part of its length. The highway was formerly part of State Route 1 and later U.S. Route 40.

SR 289 became a state highway on July 1, 1976, during the renumbering of Nevada's state highway system.

Mileposts do not correspond to actual mileage.The entire route is in Humboldt County.