State Route 353 (SR 353, OH 353) is an east–west state highway in southwestern Ohio, a U.S. state. Its western terminus is at U.S. Route 62 about 4.5 miles (7.2 km) south of Russellville, and its eastern terminus is near the unincorporated community of Decatur at State Route 125, at a point that doubles as the northern terminus of State Route 763.

This state highway runs entirely within the eastern part of Brown County. It is not included as a portion of the National Highway System.

State Route 353 was established in 1935 along the routing between U.S. Route 62 and State Route 125 that it occupies today. The highway has not experienced any major changes since its inception.

The entire route is in Brown County.