State Route 165 is a highway that runs north-to-south, with a west-to-east middle section, through portions of Dodge and Telfair counties in central Georgia. The route runs from its northern terminus at SR 46 east of Eastman to its southern terminus at SR 132 northeast of Fitzgerald.

The route runs south from its northern terminus as SR 165 for 9.5 miles (15.3 km). In Chauncey, it joins US 23, US 341 and SR 27, and is co-signed with these highways for 0.6 miles (0.97 km), running west. The route then turns southwest as SR 165 for 10.6 miles (17.1 km) into Rhine. In Rhine, it first joins SR 117, and is co-signed with this highway for 0.6 miles (0.97 km), running south. It then turns sharply east, and is co-signed with US 280 and SR 30 for 8.5 miles (13.7 km) to Milan, before turning sharply south again for 7.8 miles (12.6 km) to continue south to its southern terminus.