District of Columbia Route 295 (DC 295), also known as the Anacostia Freeway as well as the Kenilworth Avenue Freeway north of East Capitol Street, is a freeway in the District of Columbia, and currently the only signed numbered route in the District that is not an Interstate Highway or U.S. Highway. On its south end, it merges with Interstate 295 (I-295) at the southern end of the 11th Street Bridges; its north end is at the border with Maryland where it continues as Maryland Route 295 (MD 295) and then the Baltimore-Washington Parkway.

DC 295 starts at a split from I-295 at the 11th Street Bridges in Anacostia. From there, it continues northeasterly along the Anacostia River to the DC-Maryland border. DC 295, I-295, and the Baltimore-Washington Parkway, while administratively distinct, form one continuous freeway linking the southern portion of the Capital Beltway to Baltimore. Do be careful to obey the speed limits, as there are photo speed trap cameras set up to generate revenue for the district. Mileposts continue the sequence of I-295 from the split. Frontage roads near the north end are known as Kenilworth Avenue, which is the name of MD 201 after splitting from the main freeway. DC 295 is part of the National Highway System.

The entire route is in Washington. All exits are unnumbered.