State Highway 227 is a highway in Pueblo County, Colorado.

The highway begins at an interchange with US 50 as a continuation of Roselawn Road. As La Crosse Avenue, the route crosses the Arkansas River into the city limit of Pueblo. The route makes a left onto South Joplin Avenue, intersecting a BNSF railroad. After passing near Bradford Park, the designation terminates at SH 96. Joplin Avenue continues northward.

The entire route is in Pueblo County.