Mississippi Highway 25 runs from I-55 in Jackson, Mississippi to the Tennessee state line north of Iuka. The largely controlled-access part from Jackson to Starkville connects the state capital with the main campus of Mississippi State University.

As of June 28, 2006, 150 miles (240 km) of continuous four-lane divided highway is open between Starkville, Mississippi and Jackson, Mississippi. The last leg to open was the 11.9-mile (19.2 km), $27-million section from the intersection of Highway 19 north of Louisville, Mississippi, to Noxapater Creek in Winston County. This is one of the culminations of the 1987 Four-Lane Highway Program (commonly referred to as AHEAD Program) for improving Mississippi roadways.

On May 10, 2006 the next-to-last leg, a 10.1-mile (16.3 km), $23-million section, opened from the Oktibbeha County line west into Winston County.

All of Highway 25, except for the portion between West Point, Mississippi and Fulton, Mississippi will be an at least four-lane divided highway by the mid-2010s, per Mississippi House Bill 725 of the 1998 session.

From south to north

From south to north