Minnesota State Highway 27 is a highway in west-central and east-central Minnesota, which runs from its intersection with State Highway 28 in Browns Valley and continues east to its eastern terminus at its interchange with Interstate 35 in Moose Lake.

For part of its route (23 miles), Highway 27 runs concurrent with State Highway 65 in Kanabec and Aitkin counties.

Highway 27 is 248 miles (399 km) in length.

State Highway 27 serves as an east–west route between Browns Valley, Wheaton, Alexandria, Long Prairie, Little Falls, Mille Lacs Lake, and Moose Lake.

The western terminus for Highway 27 is its intersection with State Highway 28 in Browns Valley, near the Minnesota - South Dakota state line at the Little Minnesota River.

The Sam Brown Monument State Wayside Park is located at the western terminus of Highway 27 in Browns Valley.

Highway 27 crosses the Broadway Bridge at the Mississippi River in Little Falls.

Charles A. Lindbergh State Park is located immediately west of Little Falls on Highway 27.

Father Hennepin State Park is located on Highway 27 at the southeast corner of Mille Lacs Lake, one mile (1.6 km) west of Isle.

Highway 27, together with Highway 65, passes through the Solana State Forest in Aitkin County.

Most of State Highway 27 was authorized in 1933.

The original route of Highway 27 between State Highway 65 and State Highway 47 was over what is now State Highway 18; original designation of the current Highway 27 between these routes was previously old State Highway 66 until 1963.

Easternmost section of Highway 27 was previously old U.S. 61 through Moose Lake.

The section of Highway 27 southwest of Mille Lacs Lake was still gravel in 1940. The segments east of Isle were gravel into the 1950s, and the last segment east of State Highway 65 was not paved until the 1970s.