Route 16 is a primary state route connecting Middletown and Colchester via Route 66. It begins in East Hampton at Route 66 then has an interchange with Route 2 in Colchester. It then runs through Colchester center, then goes to the outskirts of Lebanon town as a rural collector road. Route 16 ends at Route 207 in Lebanon.

The western terminus of Route 16 is an intersection with Route 66 in East Hampton. It runs eastbound through East Hampton to Colchester, crossing Route 196.

In Colchester, Route 16 continues northeastward to Lebanon, crossing Route 149, connecting with Route 2, and crossing Route 85.

In Lebanon, Route 16 continues to end at an intersection with Route 207.

The portion between Route 66 and Route 85 is known as the "Henry Champion Highway".