State Route 53 (SR-53) is a 345-mile (555 km) state highway in the U.S. state of Alabama. The majority, between Dothan and Huntsville, is signed as U.S. Highway 231 (US-231), but the two ends - south to Florida and north to I-65/US-31 near Tennessee - are independent. In those areas, US-231 uses State Route 1, sharing the route with US-431 north of Huntsville, where it is referred to as Memorial Parkway.

Beginning at its southern terminus, SR-53 assumes a slight northwestwardly trajectory as it passes through Dothan, where it joins U.S. Route 231, heading towards Montgomery. Between Dothan and Huntsville, SR-53 is the unsigned partner to US-231.

At Huntsville, SR-53 diverts from US-231 and once again becomes a signed route until it reaches its terminus just west of Ardmore at Interstate 65/U.S. Highway 31 crossing several major roads along the way including University and Sparkman Drives in Huntsville. The northbound lane of SR-53 actually enters Tennessee before going south to I-65. Tennessee State Route 7 runs concurrently with the route on the state line and both Alabama and Tennessee mile markers are present on the highway.