State Route 331 is a west to east secondary highway in Knox County in the U.S. state of Tennessee.

The route is 15.4 miles (24.6 km) long. Its western terminus is in Knoxville, Tennessee at State Route 33. Its eastern terminus is in Knox County at State Route 61. The highway is known as Tazewell Pike from TN 33 (Broadway) to State Route 131 and as E Emory Road from there to TN 61.