State Route 95 (SR-95) is a 64-mile-long (103 km) route that extends from the Florida state line south of Lucy in Houston County to southern Eufaula in Barbour County.

The northern terminus of SR-95 is located at its intersection with U.S. Highway 431 to the south of downtown Eufaula. From this point, the route travels in a southwestrly direction to Abbeville. From Abbeville, the route travels in a southeasterly direction to Haleburg. From Haleburg, the route resumes a more southerly track via Columbia and Gordon en route to its southern terminus at the Florida state line. After crossing into Florida it continues as Jackson County Road 164, which was formerly Florida State Route 271.